Abstract
Lungworms (Dictyocaulus) are a type of parasitic nematode found in the lungs of vertebrates and can cause life threatening diseases to their host(s). Dictyocaulus is a type of lungworm found in cattle (Bos taurus), deer (Cervidae), goat (Capra aegagrus hircus), sheep (Ovis aries), and horses (Equus caballus). The purpose of this study was to use DNA analysis to distinguish between different species of Dictyocaulus among cattle, white-tailed deer (Odocoileus virginianus), and red deer (Cervus elaphus). Adult lungworms were collected from the lungs of cattle from research farms in Missouri, Wisconsin, and Mississippi, from the lungs of red deer from a research farm in New Zealand, and from the lungs of legally harvested white-tailed deer from Minnesota. DNA was extracted from individual lungworms using 10% Chelex. In past studies, amplification of the internal spacer 2 (ITS2) region, amplification of the major sperm protein 1 (MSP1), and cyclooxygenase (COX-1) have all been amplified via PCR and sequenced to determine the molecular difference between the species. After amplification, gel electrophoresis was then used to visualize the quality of the amplification of the desired gene. From past studies, we were able to obtain a range of species from D.v. biontis to D. eckerti using MSP1, COX-1, and ITS2. The newly sequenced DNA were aligned using Mega 10 and GenBank to determine species. ITS2 was used to identify D. eckerti in the red deer and the white-tailed deer. Additionally, the MSP-1 sequences identified D.eckerti in red deer and white-tailed deer as well. COX-1 identified that the red deer was D. viviparus whereas the white-tailed deer was D.capreolus, and D. viviparus. Preliminary data suggest that red deer and white-tailed deer may harbor the same species of lungworm.
College
College of Science & Engineering
Department
Biology
Campus
Winona
First Advisor/Mentor
Kimberly Bates
Location
Ballroom - Kryzsko Commons
Start Date
4-18-2024 10:00 AM
End Date
4-18-2024 11:00 AM
Presentation Type
Poster Session
Format of Presentation or Performance
In-Person
Session
1b=10am-11am
Poster Number
57
Included in
Molecular Methods Used to Identify a New Species of Dictyocaulus (Family Dictyocaulidae) in White-Tailed Deer
Ballroom - Kryzsko Commons
Lungworms (Dictyocaulus) are a type of parasitic nematode found in the lungs of vertebrates and can cause life threatening diseases to their host(s). Dictyocaulus is a type of lungworm found in cattle (Bos taurus), deer (Cervidae), goat (Capra aegagrus hircus), sheep (Ovis aries), and horses (Equus caballus). The purpose of this study was to use DNA analysis to distinguish between different species of Dictyocaulus among cattle, white-tailed deer (Odocoileus virginianus), and red deer (Cervus elaphus). Adult lungworms were collected from the lungs of cattle from research farms in Missouri, Wisconsin, and Mississippi, from the lungs of red deer from a research farm in New Zealand, and from the lungs of legally harvested white-tailed deer from Minnesota. DNA was extracted from individual lungworms using 10% Chelex. In past studies, amplification of the internal spacer 2 (ITS2) region, amplification of the major sperm protein 1 (MSP1), and cyclooxygenase (COX-1) have all been amplified via PCR and sequenced to determine the molecular difference between the species. After amplification, gel electrophoresis was then used to visualize the quality of the amplification of the desired gene. From past studies, we were able to obtain a range of species from D.v. biontis to D. eckerti using MSP1, COX-1, and ITS2. The newly sequenced DNA were aligned using Mega 10 and GenBank to determine species. ITS2 was used to identify D. eckerti in the red deer and the white-tailed deer. Additionally, the MSP-1 sequences identified D.eckerti in red deer and white-tailed deer as well. COX-1 identified that the red deer was D. viviparus whereas the white-tailed deer was D.capreolus, and D. viviparus. Preliminary data suggest that red deer and white-tailed deer may harbor the same species of lungworm.
