The Social Determinants of Political Behavior and Attitudes
Abstract
The research performed in this project aimed to develop a method to purify and test the enzyme PETase. His-tagged protein constructs were expressed in E. coli and purified using affinity chromatography. The protein product was quantified using Bradford assays and SDS PAGE. Plastic degradation by the protein product was also measured using absorbance at 260nm. Further applications of this research may include teaching labs and analysis of PETase mutants.
College
College of Liberal Arts
Department
Political Science & Public Administration
Campus
Winona
First Advisor/Mentor
Kara Lindaman, Ph.D.
Start Date
4-19-2023 10:00 AM
End Date
4-19-2023 11:00 AM
Presentation Type
Poster Session
Format of Presentation or Performance
In-Person
Session
1b=10am-11am
Poster Number
8
The Social Determinants of Political Behavior and Attitudes
The research performed in this project aimed to develop a method to purify and test the enzyme PETase. His-tagged protein constructs were expressed in E. coli and purified using affinity chromatography. The protein product was quantified using Bradford assays and SDS PAGE. Plastic degradation by the protein product was also measured using absorbance at 260nm. Further applications of this research may include teaching labs and analysis of PETase mutants.