There is no natural source for caffeine even though a small amount is found in groundwater. This research is designed to determine the percentage of caffeine in groundwater and try to decide which wastewater treatment process, if any, is most effective at eliminating the caffeine. In order to determine this, a method that can measure caffeine at low levels was developed. UV-visible spectrophotometry (UV-vis) was used to find the lambda max of each sample at a 10-7 g/mL dilution in order to develop a High Performance Liquid Chromatography (HPLC) method. The caffeine standards were then run using HPLC with UV-vis detection. This produced a calibration curve in which peak area was plotted against concentration. Samples will be obtained from wastewater treatment plants, run using this method and then evaluated by comparing to the standards. A solid phase extraction method will be developed and used to further purify the samples.
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