Abstract
This study aimed to experimentally determine the protein content present in various commercial protein powders. The protein that is consumed from these dietary supplements can be used to aid in the structure and function of the human body. This experimental determination of protein content present in protein powders was accomplished through the creation of both a Bradford Faculty Mentor: standard and a BCA standard. These standards were compared against the protein powder solutions created using both a Bradford and BCA assay. The actual protein content present in the protein powders of interest was then analyzed using a standard curve created from the standard it was tested against. The percent error of the protein content present in the samples were as follows in the Bradford assay were 83.5-89.5% indicating the labels overstate the protein content by nearly a factor of two. The BCA assay used confirms this with similar percent errors ranging from 81.1-101.1%.
College
College of Science & Engineering
Department
Chemistry
Campus
Winona
First Advisor/Mentor
Jonathon Mauser
Location
Ballroom - Kryzsko Commons
Start Date
4-18-2024 9:00 AM
End Date
4-18-2024 10:00 AM
Presentation Type
Poster Session
Format of Presentation or Performance
In-Person
Session
1a=9am-10am
Poster Number
25
Analysis of Protein Content in Commercial Protein Powders
Ballroom - Kryzsko Commons
This study aimed to experimentally determine the protein content present in various commercial protein powders. The protein that is consumed from these dietary supplements can be used to aid in the structure and function of the human body. This experimental determination of protein content present in protein powders was accomplished through the creation of both a Bradford Faculty Mentor: standard and a BCA standard. These standards were compared against the protein powder solutions created using both a Bradford and BCA assay. The actual protein content present in the protein powders of interest was then analyzed using a standard curve created from the standard it was tested against. The percent error of the protein content present in the samples were as follows in the Bradford assay were 83.5-89.5% indicating the labels overstate the protein content by nearly a factor of two. The BCA assay used confirms this with similar percent errors ranging from 81.1-101.1%.
