Document Type


Publication Date





This project strives to synthesize a novel dye/chitosan complex that will readily purify lactate dehydrogenase (LDH) from a homogenized chicken breast solution via affinity column chromatography. In doing so, a fiscally efficient alternative to Cibacron Blue 3-GA (CB3), a highly effective dye complex, will be elucidated. This project arose due to CB3’s price and absence from the market. Choosing an appropriate dye required the use of a molecular docking server to calculate the free binding energies (FBE) of various dyes to LDH. It was concluded that Eriochrome Black T (EBT) would most efficiently bind to LDH’s receptor site. This was surmised by comparing the EBT’s FBE of -7.83 kcal/mol to CB3’s FBE of -9.01 kcal/mol. It should also be noted that Calmagite is a worthy substitute to CB3, given its FBE of -6.67 kcal/mol. Following these calculations, a synthetic plan will be devised. The functional groups of interest are the sulfonate and sulfonic acid on EBT and Calmagite respectively. These will lend themselves to creating microwave-assisted sulfonamide linkages to chitosan. Ultimately, the successfully synthesized product(s) will be used in lieu of CB3 to provide a more affordable method to purify LDH.

Content Notes

Poster, Final Report Form, Research Report

Unique Identifier


First Advisor

Jennifer Zemke



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.