Bobcat Parasites of Wisconsin

Kryzsko Commons Ballroom, Winona, Minnesota

Bobcats (Felis rufus) are an abundant species that can be found in all areas of the United States. The species has been growing in population since 1993 when Barnes v. DNR added bobcats to the protected species list. Studies have shown that different populations of bobcats are infected with different species of parasites. Our research focused on the bobcats of Wisconsin, and identification of parasites found within their intestines. Previous research groups collected tapeworms from 115 bobcat intestines that were donated by the WI DNR from legally harvested bobcats. These were labeled by location, gender, and age. Initial identification was attempted through morphological differences after staining and mounting. These revealed subtle differences but were not conclusive. Worms that were stained and mounted were then used for molecular identification. First, DNA was extracted using 10% Chelex. We