Assessing the Bioactivity of Ruthenium β-Diketonate Complexes Through Kirby-Bauer Disk Diffusion and Monitored Real-Time Growth Assay
Presenter(s)
Dylan Wolfe
Abstract
Ruthenium(II) β-diketonate complexes present a novel approach for combating a wide range of pathogens. While similar complexes have shown some anticancer properties, their use as an antimicrobial has gone mostly unexplored. This study seeks to investigate the potential antimicrobial properties of a variety of these complexes including some curcuminoid-substituted complexes. Curcuminoids were specifically chosen as a ligand as they are analogues of curcumin, found in turmeric, which has shown some antimicrobial activity. Using Kirby-Bauer disk diffusion assays, we screened these compounds for activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Compounds exhibiting antimicrobial activity were subsequently subjected to a monitored real-time growth assay to further evaluate their bioactivity. To date, one candidate has shown promising results against E. coli and S. aureus in preliminary screenings, with additional compounds being tested. As antibiotic resistance continues to rise, identifying new therapeutic agents becomes increasingly urgent. The results from this investigation may serve as a foundation for further in vitro and in vivo studies, advancing the development of these compounds as potential drug candidates.
College
College of Science & Engineering
Department
Chemistry
Campus
Winona
First Advisor/Mentor
Jonathon Mauser
Second Advisor/Mentor
Joseph K. West
Start Date
4-24-2025 9:00 AM
End Date
4-24-2025 10:00 AM
Presentation Type
Poster Session
Format of Presentation or Performance
In-Person
Session
1a=9am-10am
Poster Number
67
Assessing the Bioactivity of Ruthenium β-Diketonate Complexes Through Kirby-Bauer Disk Diffusion and Monitored Real-Time Growth Assay
Ruthenium(II) β-diketonate complexes present a novel approach for combating a wide range of pathogens. While similar complexes have shown some anticancer properties, their use as an antimicrobial has gone mostly unexplored. This study seeks to investigate the potential antimicrobial properties of a variety of these complexes including some curcuminoid-substituted complexes. Curcuminoids were specifically chosen as a ligand as they are analogues of curcumin, found in turmeric, which has shown some antimicrobial activity. Using Kirby-Bauer disk diffusion assays, we screened these compounds for activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Compounds exhibiting antimicrobial activity were subsequently subjected to a monitored real-time growth assay to further evaluate their bioactivity. To date, one candidate has shown promising results against E. coli and S. aureus in preliminary screenings, with additional compounds being tested. As antibiotic resistance continues to rise, identifying new therapeutic agents becomes increasingly urgent. The results from this investigation may serve as a foundation for further in vitro and in vivo studies, advancing the development of these compounds as potential drug candidates.
Comments
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