Using DNA Barcoding to Determine if Minnesota Ornate Box Turtles are Native or Introduced

Presenter(s)

Jenna Bolduan, Emma Olson, and Daniel Shaughnessy

Abstract

The ornate box turtle (Terrapene ornata) is native to the southeastern U.S., with its range extending northwards into southern Wisconsin (with a disjunct population along the Wisconsin River). The ornate box turtle is not believed to exist in Minnesota, but several individuals were found in the Weaver Sand Dunes in 2024. The goal of this research is to determine if the Minnesota turtles are native or were introduced to the Weaver Sand Dunes. Partial cytochrome b (1097 bp) and cytochrome oxidase (140 bp) sequences were generated and compared to sequences of the same species across its range from GenBank to determine if the individuals found are native or introduced to Minnesota.

College

College of Science & Engineering

Department

Biology

Campus

Winona

First Advisor/Mentor

Amy Runck

Start Date

4-24-2025 10:00 AM

End Date

4-24-2025 11:00 AM

Presentation Type

Poster Session

Format of Presentation or Performance

In-Person

Session

1b=10am-11am

Poster Number

62

Comments

No poster file.

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Apr 24th, 10:00 AM Apr 24th, 11:00 AM

Using DNA Barcoding to Determine if Minnesota Ornate Box Turtles are Native or Introduced

The ornate box turtle (Terrapene ornata) is native to the southeastern U.S., with its range extending northwards into southern Wisconsin (with a disjunct population along the Wisconsin River). The ornate box turtle is not believed to exist in Minnesota, but several individuals were found in the Weaver Sand Dunes in 2024. The goal of this research is to determine if the Minnesota turtles are native or were introduced to the Weaver Sand Dunes. Partial cytochrome b (1097 bp) and cytochrome oxidase (140 bp) sequences were generated and compared to sequences of the same species across its range from GenBank to determine if the individuals found are native or introduced to Minnesota.