Detection of Carbaryl in the Southeastern Minnesota Watershed using HPLC Methods

Presenter Information

Olivia L. FunkeFollow

Presenter(s)

Jeanne Franz

Abstract

After World War II, farmers were reliant on synthetic insecticides to control insects and increase crop yield. With an ever-growing human population, the demand for food, pesticides, and fresh water has increased. One such insecticide, carbaryl, has been heavily used since its commercial introduction in the 1960s. As of 2006, carbaryl was the second most frequently detected insecticide in water and has been detected in 50% of monitored urban streams. Due to this abundance in our natural waters, methods to detect carbaryl in such water is important. A method that uses a High-Performance Liquid Chromatography, or HPLC, instrument was created. HPLC was chosen due to its high sensitivity in detecting the number, amount, and purity of pollutants in water. A calibration curve and internal standard was constructed on the HPLC instrument. This was done by creating solutions of carbaryl and methanol in increasing concentrations of 2.5, 5, 7, and 10 ng/mL respectively. The internal standard chosen was naphthol because it would not normally be found in the environment and its peak appeared at a drastically different time than carbaryl. Carbaryl was found to display a peak at about 5.5 minutes and naphthol had a peak at about 8 minutes. To test carbaryl in natural water, integrating a method using Solid Phase Extraction is needed and still must be created. These steps were taken to hopefully determine if carbaryl exists in natural water in the Southeastern Minnesota watershed.

College

College of Science & Engineering

Department

Chemistry

Campus

Winona

Start Date

4-24-2025 1:00 PM

End Date

4-24-2025 2:00 PM

Presentation Type

Poster Session

Format of Presentation or Performance

In-Person

Session

2a=1pm-2pm

Poster Number

15

Comments

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Apr 24th, 1:00 PM Apr 24th, 2:00 PM

Detection of Carbaryl in the Southeastern Minnesota Watershed using HPLC Methods

After World War II, farmers were reliant on synthetic insecticides to control insects and increase crop yield. With an ever-growing human population, the demand for food, pesticides, and fresh water has increased. One such insecticide, carbaryl, has been heavily used since its commercial introduction in the 1960s. As of 2006, carbaryl was the second most frequently detected insecticide in water and has been detected in 50% of monitored urban streams. Due to this abundance in our natural waters, methods to detect carbaryl in such water is important. A method that uses a High-Performance Liquid Chromatography, or HPLC, instrument was created. HPLC was chosen due to its high sensitivity in detecting the number, amount, and purity of pollutants in water. A calibration curve and internal standard was constructed on the HPLC instrument. This was done by creating solutions of carbaryl and methanol in increasing concentrations of 2.5, 5, 7, and 10 ng/mL respectively. The internal standard chosen was naphthol because it would not normally be found in the environment and its peak appeared at a drastically different time than carbaryl. Carbaryl was found to display a peak at about 5.5 minutes and naphthol had a peak at about 8 minutes. To test carbaryl in natural water, integrating a method using Solid Phase Extraction is needed and still must be created. These steps were taken to hopefully determine if carbaryl exists in natural water in the Southeastern Minnesota watershed.